- The affinity is between KN & KT suction created by the link. if force becomes strong 3-dimensional structure between KT and epitop position as fits together
- agglutination reaction: a reaction between the tangible and the KN KN dissolved. The KN usually red blood cells or the antigen of VSV. End the possible indirect or passive agglutination
First KN must soluble surface-mounted inert substances (latex particles, betonit ..). The granules dissolve after being surrounded KN will react as if they carry the specificity of the KN
- agglutination reaction with complement
activation pathways In complement classic combination KN, KT and complement. If antigen specificity with KT will combine to complement, and not specific to the complement KT in the free state, not combined
- ELISA technique
This technique is simple and fairly sensitive to allow determine KN, KT in low concentrations. The principle of this technique is equivalent to pp immunofluorescence: KT tied with 1 enzyme identified, adding the substrate to the reaction occurs; hydrolyzing enzyme substrate compounds produce color; said color intensity concentration of KN, KT should determine
+ ELISA technique can be modified to direct technical or indirectly into other types of enzymes: glucocidaza, perocidaza, photphadaza alkaline
+ - + Basic Principles :
According to the basic principles of immunology, each one antigen (antigen) are more antigenic determinants (epitopes), each one epitope capable of combining with an antibody (antibody) corresponding to it. Most antigens are one or several epitopes specific to it, based on which one can identify the antigens. Besides, the presence of antigens in the body in some cases have the ability to stimulate the body produce monoclonal antibodies specific against that antigen. Through identifying human antibodies can also indirectly determine the antigen in the body.
Based on that, ELISA was established to diagnose the presence of an antigen or antibody. To determine a factor to diagnosis (antigen / antibody) were used one or more elements detected (antibody / antigen / complement) have specific immune response to the factors to diagnosis . Factors findings are marked by the enzyme so that the immune response to the factors to diagnosis will make a change can be identified with the naked eye or even quantified by tools other than color when the substrate of the enzyme tick.
- PP immunofluorescence
is used or KN KT is marked with fluorescence-based nature of the dye is excited by radiation having a wavelength-specific, it will glow
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