Tooptimizetheproportionofcarbonandnitrogensources, andthecarbonnitrogenratioduringsubmergedfermenta tionofAgaricusbisporus,
. Andtoprovidereferencesfortheconservationanddomesticationofwildgermplasm [phương pháp] Atotalof7organic nitrogensourcesand4inorganicnitrogensourceswereoptimizedandcompared.Thepelletdiameter, pelletdensity, myceliumbiomassand otherindiceswereresearched.ThenitrogenmetabolismcharacteristicsofA.bisporusundersubmergedfermentationcultureconditionwerein vestigated. [Kết quả] Theoptimalcombinationofcarbonandnitrogensourcesandinorganicsaltsforthesubmergedfermentationcultureof A.bisporuswasselectedbyorthogonaltest; andtheoptimalculturemediumwas3 glucose%, 3% xylose, 0,3% peptone, 5 % milletsprout, 0,1% KH2PO4,0.05% MgSO 4 · 7H2O, 0,2% (NH4) 2SO4, pH5.5and35 ℃ .Thenitrogensourceandconcentrationwereselected, nào significantlyorextremelyimprovedthegrowthofA.bisporus.milletandmilletsproutculturemediawerethebestnitrogensourcesforslantcul tureofA.bisporus.Themyceliumpelletdensitiesofmilletandmilletsproutculturemediareached27.64% and25. 00%, tương ứng, nào were162% and142% ofthecontrol.Peptoneandyeastextractsshowedsignificantdifferences.However, Glycine, ammoniumsulfateandpo tassiumnitrateshowednosignificantdifferences.Andureawastheworstnitrogensource.3.1% milletandmilletsproutcouldbothproduce themaximumbiomass, sotheycouldtaketheplaceofpeptoneandyeastextracts.TheoptimalcarbonnitrogenratioforthegrowthofA.bis poruswas35:. 1 [Kết luận] TherewereselectivedifferencesinthenitrogensourceofA.bisporus.Organicnitrogensourcewassuperiorto othersources .Andthenitrogenconcentrationhadextremelysignificanteffectsontheyieldofslantculture. Từ khóa Agaricusbisporus; Slantculture; Nitrogensource; Carbonnitrogenrat
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